SciCombinator

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Journal: Cellular and molecular biology (Noisy-le-Grand, France)

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To analyze the effects of blastocysts on the 5th day (D5) and 6th day (D6) of frozen-thawed blastocyst transplantation on pregnancy outcome and provide evidence for further improvement of the strategy. This study included transfers from the Reproductive Medicine Center of the Second Affiliated Hospital of Wenzhou Medical University during freeze-thaw cycles from January 2016 to December 2017. They were divided into D5 group (1616 cases) and D6 group (619 cases) according to blastocyst formation and development. Each group was further divided into 5 groups according to the quality of the blastocyst and the number of transplants, making a total of 10 groups. Following the frozen transplantation cycle, the transplanting rate was significantly higher for D5 (41.73%) than for D6 (23.98%) (P < 0.05); the ongoing pregnancy rate (47,40%) was also significantly higher than that of D6 (28.43%) (P < 0.05).In the frozen-thawed blastocyst resuscitation transplantation, compared to D6 blastocysts, D5 blastocysts were more conducive to blastocyst implantation and could be used to achieve better clinical pregnancy outcome. In blastocyst selection, a single D5 excellent blastocyst transplant is preferred. Only at the 6th day of non-excellent D6, 2 blastocysts are recommended for transplantation.

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In order to estimate the efficiency of SRAP markers for identifying the performance of seed quantity and quality in maize single crosses, 13 inbred lines obtained from CIMMYT germplasm bank were crossed to A679, K166B, K18 and MO17 testers using the line×tester method. The inbred lines and derived hybrids were evaluated in two experiments separately in a randomized complete block design with three replications during two growing seasons in 2014 and 2015. In order to evaluate genetic variation in the inbred lines, 25 SRAP markers were also used. The results of variance analysis between inbred lines were showed a significant variation (P≤0.01) for seed quantity and quality. The analysis of variance among the hybrids derived from inbred lines was showed a significant variation (P≤0.01) for oil percent, starch content, protein content, seed yield and thousand seed weights and a significant variation for the dry matter (P≤0.05). The maximum Euclidian distance between the two lines was 24.5 times greater than the minimum distance between two lines. The PCR amplification for the 17 parentallines with the 25 combinations of SRAP primers generated a total of 205 clear and scorable bands, of which 135 were polymorphic (65.75%). The average distance between the studied lines was 0.324 on the bases of the Jaccard coefficient and maximum distance between two lines was 2.87 times greater than the minimum distance between two lines. The M1E1, M1E5, M5E3, M5E4, and M5E5 were superior to other primer combinations in expressing genetic diversity based on the primer information indices. The banding pattern of the studied primer combinations related to the genetic variation of the inbred lines based on the studied traits revealed that the M5E1 primer pair can predict the distance of inbred lines for dry matter better than other primers. Also, the primers combination of M4E4 for protein percentage, M4E4 for starch percentage, M2E3 for crude fiber, M4E3 for oil percentage, M2E5, M4E1 and M5E1 for thousand seed weight and M3E1 for seed yield, can be introduced as informative primer combination, to estimate genetic distance determination of inbred lines based on these traits. Due to the relationship between inbred lines variation based on primers combinations with the traits in hybrids progenies showed that the M2E1, M2E2, M4E1 and M5E3 for dry matter and M2E4 for starch percentage have the ability to detect hybrid performance for these traits. For traits, protein percentage, crude fiber and oil percent no suitable primers combination were found. Also, for the seed yield, three primer combinations of M1E5, M2E2 and M3E2 had the highest negative correlation. Therefore, the hybrids derived from the inbred lines with high genetic distance based on these primers combinations will have a low seed yield. The M1E2, M2E3 and M5E5 can be introduced to identify the prediction of higher thousand seed weight.

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Candida species are considered as one of the important cause of nosocomial and community infections. Candidacies are fourth caused by septicemia in some countries and possess extra cost to the health care system. The aim of this study was survey the presence of virulence factors associated with various candida geniuses in samples which have been collected from the intensive care unit. In this cross-sectional study, various clinical specimens have been collected from patients which hospitalized in the Intensive Care Unit (ICU) of Milad hospital, Tehran, Iran. The species of candida has been determined by CHROM agar. Finally, adherence factors genes and proteinase gene have been detected by PCR. In this research, 100 samples have been collected from patients that colonized with candida. C. albicans (63%) and C.glabrata (19.4%) are the most identified species, respectively. The species of four specimens have been not detected according to the color of CHROM agar candida medium and two different genus of candida has been isolated from 7 patients. The frequency of Als1, Als3, HWP1 and SAP1 genes among C. albicans was (92%), (94%), (95%) and (88%), respectively. The most detected virulence factor was HWP1 and SAP4 was the lowest one. At least two virulence factors have been detected in 95% of different Candida species that can cause invasive fungal properties. These results are important for infection control committee in the hospital because invasive fungal diseases can make a serious problem for patients that hospitalized in ICU.

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In recent years, most related studies have found that chronic hepatitis B virus infection is the main cause of hepatocellular carcinoma (HCC), but the specific pathogenesis is still unclear. To investigate the function of HDAC in hepatocellular carcinoma (HCC), this study used qRT-PCR to determine the expression levels of miR-376a and HDAC9 mNRA in HCC and para-cancerous tissues. The clinical significance of HDAC9 in HCC was assessed in a study cohort containing 37 patients with HCC using immunohistochemistry. The expression level of miR-376a in liver cancer tissues was significantly lower than that in para-cancerous tissues, while the expression level of HDAC9 mRNA in liver cancer tissue was significantly higher than that in para-cancerous tissues. The expression of HDAC9 occurred mainly in the nucleus. There was a significant correlation between tumor differentiation and HDAC9. Survival analysis showed that HCC patients with higher HDAC9 expression had poorer prognosis, and subsequent multivariate analysis showed that HDAC9 expression level was an independent predictor. There was a definite correlation between HDAC9 and the expressions of AFP and Ki67. These results suggest that the expression level of HDAC9 in HCC is abnormally high while the expression level of miR-376a is significantly decreased, indicating that HDAC9 may be a potential prognostic indicator of HCC.

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Concerns about nanoparticles environmental pollution risk have been increased globally due to an increase in the production of nanoparticles in recent years and their use in diverse cases. The purpose of this experiment was to study the alleviation effect of humic acid on nanoparticles toxicity in greenhouse conditions. Thus two separate experiments were conducted at the rosette growing stages of rapeseed in a factorial experiment as a completely randomized design with three replications. The first factor was copper and zinc oxide nanoparticle in five concentrations of 0, 500, 1000, 1500, 2000 mg.L-1 in each of experiments and the second factor was humic acid in two concentrations of 0 and 100 mg.L-1 in both experiments. The results showed that simultaneously application of humic acid and the nanoparticles resulted in increasing of chlorophyll, protein contents, and antioxidants enzymes activity. For example, the maximum activity of catalase was 170.72 and 296.82 µmol.min-1.mg-1 proteins when CuO nanoparticle was utilized alone and together with humic acid respectively. Also increasing the concentration of CuO nanoparticle reduced protein content from 2.44 to 1.88 (mg.gr-1 Fresh leaf weight), while its range was 2.86 and 2.49 (mg.gr-1 Fresh leaf weight) when adding the humic acid. Transmission electron microscopy images of root tissue confirm the decreasing of nanoparticles entrance to plant cell and tissue by humic acid. In general, application of humic acid alleviated the nanoparticles toxicity, due to the high adsorption capacity that is able to get out the metals from plants or like-hormonal activity probably.

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The immune system is an important physiological defense system. Its balance and stability are closely related to the body’s health. Once the immune system loses its dynamic balance, the immune response will be blocked, which will lead to the occurrence of various diseases. Hesperetin is a kind of natural flavonoids extracted from citrus fruits of Rutaceae and it has many pharmacological activities. However, its water solubility and liposolubility are poor, and it is easy to be quickly metabolized in vivo, so it is difficult to maintain high blood drug concentration. Therefore, its derivative (HES) was found by structural modification. In this study, THP-1 cells were used as experimental model to investigate the immunomodulatory effect of HES in vitro. The results showed that HES participates in immune response by enhancing phagocytosis of macrophages to promote the release of NO, IL-6 and IL-1β, and enhancing immunity by up-regulating the expression of Bcl-2 and Bcl-XL proteins. This study provides a theoretical and practical basis for the development of HES as an immunomodulator in the future.

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Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related deaths. Compound K, an active metabolite of ginsenosides, is reported to exhibit anti-cancer property in various types of human malignancies. The present study investigated the role of compound K on glucose metabolism in NSCLC cells and its underlying mechanism. Our study found that compound K dose-dependently inhibited the cell viability of NSCLC cells. Moreover, administration with compound K decreased glucose uptake and lactate secretion under normoxic and hypoxic conditions. Consistently, the expression of key enzymes (HK II, PDK1 and LDHA) involved in glucose metabolism were inhibited in compound K-treated tumor cells. In addition, compound K inhibited the expression of HIF-1α and its downstream gene GLUT1. On the contrary, overexpression of HIF-1α elevated metabolic reactions and partly attenuated the inhibitory role of compound K on NSCLC cell growth. These results demonstrate that compound K suppresses NSCLC cell growth via HIF-1α mediated metabolic alteration, contributing to novel anticancer therapy by targeting glucose metabolism.

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Thyroid hormones regulate the development and maturation of the brain by maintaining levels of neurotransmitters and their related metabolites. The present work emphasizes the neural dysfunction in the brain caused by hypothyroidism and the potential role of Hordeum vulgare (water soluble barley, (B)) in ameliorating these effects. The study was conducted on euothyroid and hypothyroid adult female rats. The induction of hypothyroidism was conducted by oral-administration of neo-mercazole (5.0 mg.kg-1) daily for thirty days prior the study and terminated at the end of the study. The groups were assigned as; euthyroid (EU) and hypothyroid (H) groups and other two groups were treated with 100 mg.kg-1 water soluble barley; daily for one month and assigned as (EU+B) and (H+B) groups. Compared with EU and EU+B groups, a reduction in fT4, and ERK1/2 levels and elevation in TSH in brain tissue, Moreover, a  significant elevation in 8-OH deoxyguanosine and caspase-3 levels, confirmed with increase percentage DNA-damage in the brain and thyroid tissues in hypothyroid control rats. Furthermore, a significant decrease in all monoamines levels in different brain areas and downregulation of dopamine and 5-hydroxytreptamin receptors transcription, with a significant increase in excitatory amino acids and no significant change in the levels inhibitory amino acids were recorded in control hypothyroid group. Treatment of hypothyroid group with Hordeum vulgare improved the above-mentioned adverse impact by ameliorating the thyroid hormone levels with depleting the DNA-degradation and elaborating the levels of neurotransmitters with related receptors and amino acids in brain areas.  Water soluble Hordeum vulgare as a phytonutrient, is safe and efficient agent in ameliorating the neural dysfunction resulting from hypothyroidism status in adult female rats.

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To investigate the expressions and roles of semaphorin3A (Sema3A) and vascular endothelial growth factor 165 (VEGF165) in cultured rat cortical neurons and vascular endothelial cells after oxygen glucose deprivation (OGD) stimulation. Cultured cortical neurons (NC) and vascular endothelial cells (VEC) of Sprague Dawley (SD) rats (SPF grade) were randomly divided into control group and OGD treatment group. Western blot assay, immunofluorescent staining and immunohistochemical methods were used to determine the expressions of VEGF165, Sema3A and neuropilin-1 (Nrp-1) protein. Cell migration was determined by Transwell, while TUNEL assay was used to measure apoptosis. The expressions of Sema3A, Nrp-1 and VEGF165 in NC and VEC cells after OGD treatment were up-regulated, when compared with the control group. With transfection of Sema3A shRNA, apoptosis of neurons decreased significantly after 2 h of OGD treatment, but the apoptosis of VEC cells was not obvious. The migration rate of VEC cells in the treatment group was significantly increased, relative to that of the control group. Stimulation with OGD induces neuronal expression of VEGF165 and regulates the migration of vascular endothelial cells, thereby enhancing their participation in angiogenesis, which may involve Sema3A.

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Effect of ginkgetin on proliferation of human cervical cancer (HeLa) cells and the underlying mechanism   were investigated. Human cervical cancer (HeLa) cells were cultured at 37 °C in 10 % fetal bovine serum (FBS) supplemented RPMI 1640 medium in a humidified incubator containing 5 % CO2. Cell proliferation was determined using MTT assay, while real-time quantitative polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to determine the levels of expression of interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin 8 (IL-8). The expressions of p38 mitogen-activated protein kinases (p38 MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF- κB) were determined using Western blotting. Treatment of HeLa cells with ginkgetin significantly and time- and dose-dependently inhibited their proliferation (p < 0.05). The invasion of the cells were also significantly and dose-dependently decreased, when compared with control cells (p < 0.05). The expressions of p-p38 and p-NF-κB were significantly and dose-dependently down-regulated, relative to control group (p < 0.05). However, the expressions of p38 and NF-κB in ginkgetin-treated cells were not significantly different from those of control group (p > 0.05). The results of qRT-PCR and ELISA showed that the levels of expression of TNF-α, IL-1β and IL-8 mRNAs were significantly and dose-dependently reduced in HeLa cells after 48 h of treatment with ginkgetin, when compared with the control group (p < 0.05). The anti-proliferative effect of ginkgetin on HeLa cells is exerted via a mechanism involving the p38/NF-κB pathway.