Scanning small and wide angle X-ray scattering (scanning SWAXS) experiments were performed on healthy and pathologic human bone sections. Via crystallographic tools the data were transformed into quantitative images and as such compared with circularly polarized light (CPL) microscopy images. SWAXS and CPL images allowed extracting information of the mineral nanocrystalline phase embedded, with and without preferred orientation, in the collagen fibrils, mapping local changes at sub-osteon resolution. This favorable combination has been applied for the first time to biopsies of dwarfism syndrome and Paget’s disease to shed light onto the cortical structure of natural bone in healthy and pathologic sections.
Optical metamaterials are usually based on planarized, complex-shaped, resonant nano-inclusions. Three-dimensional geometries may provide a wider set of functionalities, including broadband chirality to manipulate circular polarization at the nanoscale, but their fabrication becomes challenging as their dimensions get smaller. Here we introduce a new paradigm for the realization of optical metamaterials, showing that three-dimensional effects may be obtained without complicated inclusions, but instead by tailoring the relative orientation within the lattice. We apply this concept to realize planarized, broadband bianisotropic metamaterials as stacked nanorod arrays with a tailored rotational twist. Because of the coupling among closely spaced twisted plasmonic metasurfaces, metamaterials realized with conventional lithography may effectively operate as three-dimensional helical structures with broadband bianisotropic optical response. The proposed concept is also shown to relax alignment requirements common in three-dimensional metamaterial designs. The realized sample constitutes an ultrathin, broadband circular polarizer that may be directly integrated within nanophotonic systems.
Optical telecommunication employs light pulses travelling down optical fibres; in a binary format logical Ones and Zeroes are represented by the presence or absence of a light pulse in a given time slot, respectively. The fibre’s data-carrying capacity must keep up with increasing demand, but for binary coding it now approaches its limit. Alternative coding schemes beyond binary are currently hotly debated; the challenge is to mitigate detrimental effects from the fibre’s nonlinearity. Here we provide proof-of-principle that coding with solitons and soliton molecules allows to encode two bits of data per clock period. Solitons do not suffer from nonlinearity, rather, they rely on it; this endows them with greater robustness. However, they are universally considered to be restricted to binary coding. With that notion now refuted, it is warranted to rethink future systems.
Wherever the polarization properties of a light beam are of concern, polarizers and polarizing beamsplitters (PBS) are indispensable devices in linear-, nonlinear- and quantum-optical schemes. By the very nature of their operation principle, transformation of incoming unpolarized or partially polarized beams through these devices introduces large intensity variations in the fully polarized outcoming beam(s). Such intensity fluctuations are often detrimental, particularly when light is post-processed by nonlinear crystals or other polarization-sensitive optic elements. Here we demonstrate the unexpected capability of light to self-organize its own state-of-polarization, upon propagation in optical fibers, into universal and environmentally robust states, namely right and left circular polarizations. We experimentally validate a novel polarizing device - the Omnipolarizer, which is understood as a nonlinear dual-mode polarizing optical element capable of operating in two modes - as a digital PBS and as an ideal polarizer. Switching between the two modes of operation requires changing beam’s intensity.
The coupling between DNA molecules and quantum dots can result in impressive nonlinear optical properties. In this paper, we theoretically demonstrate the significant enhancement of Kerr coefficient of signal light using optical pump-probe technique when the pump-exciton detuning is zero, and the probe-exciton detuning is adjusted properly to the frequency of DNA vibration mode. The magnitude of optical Kerr coefficient can be tuned by modifying the intensity of the pump beam. It is shown clearly that this phenomenon cannot occur without the DNA-quantum dot coupling. The present research will lead us to know more about the anomalous nonlinear optical behaviors in the hybrid DNA-quantum dot systems, which may have potential applications in the fields such as DNA detection.
Ecological photopollution created by artificial night lighting can alter animal behavior and lead to population declines and biodiversity loss. Polarized light pollution is a second type of photopollution that triggers water-seeking insects to ovisposit on smooth and dark man-made objects, because they simulate the polarization signatures of natural water bodies. We document a case study of the interaction of these two forms of photopollution by conducting observations and experiments near a lamp-lit bridge over the river Danube that attracts mass swarms of the mayfly Ephoron virgo away from the river to oviposit on the asphalt road of the bridge. Millions of mayflies swarmed near bridge-lights for two weeks. We found these swarms to be composed of 99% adult females performing their upstream compensatory flight and were attracted upward toward unpolarized bridge-lamp light, and away from the horizontally polarized light trail of the river. Imaging polarimetry confirmed that the asphalt surface of the bridge was strongly and horizontally polarized, providing a supernormal ovipositional cue to Ephoron virgo, while other parts of the bridge were poor polarizers of lamplight. Collectively, we confirm that Ephoron virgo is independently attracted to both unpolarized and polarized light sources, that both types of photopollution are being produced at the bridge, and that spatial patterns of swarming and oviposition are consistent with evolved behaviors being triggered maladaptively by these two types of light pollution. We suggest solutions to bridge and lighting design that should prevent or mitigate the impacts of such scenarios in the future. The detrimental impacts of such scenarios may extend beyond Ephoron virgo.
The purpose of this study was to observe in vitro matured equine oocytes with an objective computerized technique which involve the use of a polarized light microscope (PLM) in addition to the subjective morphological evaluation obtained using a classic light microscope (LM). Equine cumulus-oocyte complexes (COCs, n=922) were subjected to different in vitro maturation times (24, 36, or 45 h), but only 36-h matured oocytes were analyzed using PLM. The 36-h matured oocytes that reached maturity were parthenogenetically activated to evaluate quality and meiotic competence. Average maturation percentages per session in groups 1, 2, and 3 (24-, 36- and 45-h matured oocytes, respectively) were 29.31±13.85%, 47.01±9.90%, and 36.62±5.28%, whereas the average percentages of immature oocytes per session were 28.78±20.17%, 7.83±5.51%, and 22.36±8.39%, respectively. The zona pellucida (ZP) birefringent properties were estimated and correlated with activation outcome. ZP thickness and retardance of the inner layer of the zona pellucida (IL-ZP) were significantly increased in immature oocytes compared with mature oocytes (p<0.001 and p<0.01, respectively). The comparison between parthenogenetically activated and non-activated oocytes showed a significant increase in the area and thickness of the IL-ZP in parthenogenetically activated oocytes (p<0.01). These results show that the 36-h IVM protocol allowed equine oocytes to reach maturity, and PLM observation of ZP can be used to distinguish mature and immature oocytes as well as activated and non-activated oocytes.
Although macrophages can be polarized to distinct phenotypes in vitro with individual ligands, in vivo they encounter multiple signals that control their varied functions in homeostasis, immunity, and disease. Here, we identify roles of Rev-erb nuclear receptors in regulating responses of mouse macrophages to complex tissue damage signals and wound repair. Rather than reinforcing a specific program of macrophage polarization, Rev-erbs repress subsets of genes that are activated by TLR ligands, IL4, TGFβ, and damage-associated molecular patterns (DAMPS). Unexpectedly, a complex damage signal promotes co-localization of NF-κB, Smad3, and Nrf2 at Rev-erb-sensitive enhancers and drives expression of genes characteristic of multiple polarization states in the same cells. Rev-erb-sensitive enhancers thereby integrate multiple damage-activated signaling pathways to promote a wound repair phenotype.
Optical fibre underpins the global communications infrastructure and has experienced an astonishing evolution over the past four decades, with current commercial systems transmitting data rates in excess of 10 Tb/s over a single fibre core. The continuation of this dramatic growth in throughput has become constrained due to a power dependent nonlinear distortion arising from a phenomenon known as the Kerr effect. The mitigation of fibre nonlinearities is an area of intense research. However, even in the absence of nonlinear distortion, the practical limit on the transmission throughput of a single fibre core is dominated by the finite signal-to-noise ratio (SNR) afforded by current state-of-the-art coherent optical transceivers. Therefore, the key to maximising the number of information bits that can be reliably transmitted over a fibre channel hinges on the simultaneous optimisation of the modulation format and code rate, based on the SNR achieved at the receiver. In this work, we use an information theoretic approach based on the mutual information and the generalised mutual information to characterise a state-of-the-art dual polarisation m-ary quadrature amplitude modulation transceiver and subsequently apply this methodology to a 15-carrier super-channel to achieve the highest throughput (1.125 Tb/s) ever recorded using a single coherent receiver.
Colloidal-scale assemblies that reconfigure on demand may serve as the next generation of soft “microbots,” artificial muscles, and other biomimetic devices. This requires the precise arrangement of particles into structures that are preprogrammed to reversibly change shape when actuated by external fields. The design and making of colloidal-scale assemblies with encoded directional particle-particle interactions remain a major challenge. We show how assemblies of metallodielectric patchy microcubes can be engineered to store energy through magnetic polarization and release it on demand by microscale reconfiguration. The dynamic pattern of folding and reconfiguration of the chain-like assemblies can be encoded in the sequence of the cube orientation. The residual polarization of the metallic facets on the microcubes leads to local interactions between the neighboring particles, which is directed by the conformational restrictions of their shape after harvesting energy from external magnetic fields. These structures can also be directionally moved, steered, and maneuvered by global forces from external magnetic fields. We illustrate these capabilities by examples of assemblies of specific sequences that can be actuated, reoriented, and spatially maneuvered to perform microscale operations such as capturing and transporting live cells, acting as prototypes of microbots, micromixers, and other active microstructures.