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Concept: Manure


Increasing diffuse nitrate loading of surface waters and groundwater has emerged as a major problem in many agricultural areas of the world, resulting in contamination of drinking water resources in aquifers as well as eutrophication of freshwaters and coastal marine ecosystems. Although empirical correlations between application rates of N fertilizers to agricultural soils and nitrate contamination of adjacent hydrological systems have been demonstrated, the transit times of fertilizer N in the pedosphere-hydrosphere system are poorly understood. We investigated the fate of isotopically labeled nitrogen fertilizers in a three-decade-long in situ tracer experiment that quantified not only fertilizer N uptake by plants and retention in soils, but also determined to which extent and over which time periods fertilizer N stored in soil organic matter is rereleased for either uptake in crops or export into the hydrosphere. We found that 61-65% of the applied fertilizers N were taken up by plants, whereas 12-15% of the labeled fertilizer N were still residing in the soil organic matter more than a quarter century after tracer application. Between 8-12% of the applied fertilizer had leaked toward the hydrosphere during the 30-y observation period. We predict that additional exports of (15)N-labeled nitrate from the tracer application in 1982 toward the hydrosphere will continue for at least another five decades. Therefore, attempts to reduce agricultural nitrate contamination of aquatic systems must consider the long-term legacy of past applications of synthetic fertilizers in agricultural systems and the nitrogen retention capacity of agricultural soils.

Concepts: Agriculture, Water, Fertilizer, Eutrophication, Soil, Nitrogen, Phosphorus, Manure


Debate exists about whether agricultural versus medical antibiotic use drives increasing antibiotic resistance (AR) across nature. Both sectors have been inconsistent at antibiotic stewardship, but it is unclear which sector has most influenced acquired AR on broad scales. Using qPCR and soils archived since 1923 at Askov Experimental Station in Denmark, we quantified four broad-spectrum β-lactam AR genes (ARG; blaTEM, blaSHV, blaOXA and blaCTX-M) and class-1 integron genes (int1) in soils from manured (M) versus inorganic fertilised (IF) fields. “Total” β-lactam ARG levels were significantly higher in M versus IF in soils post-1940 (paired-t test; p < 0.001). However, dominant individual ARGs varied over time; blaTEM and blaSHV between 1963 and 1974, blaOXA slightly later, and blaCTX-M since 1988. These dates roughly parallel first reporting of these genes in clinical isolates, suggesting ARGs in animal manure and humans are historically interconnected. Archive data further show when non-therapeutic antibiotic use was banned in Denmark, blaCTX-M levels declined in M soils, suggesting accumulated soil ARGs can be reduced by prudent antibiotic stewardship. Conversely, int1 levels have continued to increase in M soils since 1990, implying direct manure application to soils should be scrutinized as part of future stewardship programs.

Concepts: Bacteria, Agriculture, Antibiotic resistance, Soil, Antibiotic, Antibiotics, Manure, Alternate reality game


Steroid oestrogens (SE) are released by humans and animals into the environment. In the Mekong Delta animal excrement is directly discharged into surface water and can pollute the water. Only a few animal production sites are currently treating the excrement in either biogas plants or vermicomposting systems. The concentration of SE in manures from pigs and cattle was monitored in the Mekong Delta, Vietnam. Fresh cow faeces had an oestrogen concentration of 3.3 ng E2 eq/g dry weight. The SE concentration in effluent from biogas plants fed with animal manures was 341 ng E2 eq/L. Most of the SE were in the solid phase (77.9-98.7%). Vermicomposting reduced SE to 95% of the original input.

Concepts: Photosynthesis, Plant, Cattle, Anaerobic digestion, Feces, Compost, Manure, Cow dung


Anaerobic digestion of residual materials from animals and crops offers an opportunity to simultaneously produce bioenergy and plant fertilizers at single farms and in farm communities where input substrate materials and resulting digested residues are shared among member farms. A surplus benefit from this practice may be the suppressing of propagules from harmful biological pests like weeds and animal pathogens (e.g. parasites). In the present work, batch experiments were performed, where survival of seeds of seven species of weeds and non-embryonated eggs of the large roundworm of pigs, Ascaris suum, was assessed under conditions similar to biogas plants managed at meso- (37°C) and thermophilic (55°C) conditions. Cattle manure was used as digestion substrate and experimental units were sampled destructively over time. Regarding weed seeds, the effect of thermophilic conditions (55°C) was very clear as complete mortality, irrespective of weed species, was reached after less than 2 days. At mesophilic conditions, seeds of Avena fatua, Sinapsis arvensis, Solidago canadensis had completely lost germination ability, while Brassica napus, Fallopia convolvulus and Amzinckia micrantha still maintained low levels (∼1%) of germination ability after 1 week. Chenopodium album was the only weed species which survived 1 week at substantial levels (7%) although after 11d germination ability was totally lost. Similarly, at 55°C, no Ascaris eggs survived more than 3h of incubation. Incubation at 37°C did not affect egg survival during the first 48h and it took up to 10days before total elimination was reached. In general, anaerobic digestion in biogas plants seems an efficient way (thermophilic more efficient than mesophilic) to treat organic farm wastes in a way that suppresses animal parasites and weeds so that the digestates can be applied without risking spread of these pests.

Concepts: Archaea, Plant, Anaerobic digestion, Biogas, Sintex Digester, Manure, Thermophile, Mesophile


This study sought to evaluate the efficacy of aerobic and anaerobic composting of inoculated banana peels, and assess the agronomic value of banana peel-based compost. Changes in the chemical composition under aerobic and anaerobic conditions were examined for four formulations of banana peel-based wastes over a period of 12weeks. The formulations i.e. plain banana peel (B), and a mixture with either cow dung (BC), poultry litter (BP) or earthworm (BE) were separately composted under aerobic and anaerobic conditions under laboratory conditions. Inoculation with either cow dung or poultry litter significantly facilitated mineralization in the order: BP>BC>B. The rate of decomposition was significantly faster under aerobic than in anaerobic composting conditions. The final composts contained high K (>100gkg(-1)) and TN (>2%), indicating high potential as a source of K and N fertilizer.

Concepts: Nitrogen, Waste management, Humus, Compost, Composting, Manure, Anaerobic exercise, Vermicompost


ETHNOPHARMACOLOGICAL RELEVANCE: Red edible bird’s nests are regarded as of higher beneficial value for health and hence fetch a higher price than the white ones. Their red colour remains a myth. AIM OF THE STUDY: To determine if white edible bird’s nests can turn red by vapours generated from sodium nitrite in acidic conditions and by vapours from ‘bird soil’. MATERIALS AND METHODS: White edible bird’s nests were exposed to vapours from sodium nitrite dissolved in 2% HCl or from ‘bird soil’ in hot and humid conditions. CONCLUSIONS: Vapours from sodium nitrite dissolved in 2% HCl or from ‘bird soil’ containing guano droppings from swiftlet houses were able to turn white edible bird’s nests red. The reddening agent in ‘bird soil’ was water-soluble and heat-stable. The red colour of edible bird’s nests is likely caused by the environmental factors in cave interiors and swiftlet houses.

Concepts: Ammonia, Color, Green, Red, White, Primary color, Guano, Manure


We developed a quantitative real-time PCR assay to specificity detect and quantify the genus Alcaligenes in samples from the agricultural environment, such as vegetables and farming soils. The minimum detection sensitivity was 106 fg of pure culture DNA, corresponding to DNA extracted from two cells of A. faecalis. To evaluate the detection limit of A. faecalis, serially diluted genomic DNA from A. faecalis was mixed with DNA extracted from soil and vegetables, and then a standard curve was constructed. These results indicated that Alcaligenes species are present in the plant phytosphere at levels 10(2) -10(4) times lower than those in soil. Our approach will be useful for tracking or quantifying species of the genus Alcaligenes in the agricultural environment.

Concepts: DNA, Gene, Biodiversity, Polymerase chain reaction, Organism, Species, Manure, Alcaligenes faecalis


Repeated applications of animal manure as fertilizer are normal agricultural practices that may release veterinary antibiotics and hormones into the environment from treated animals. Broiler manure samples and their respective manure-amended agricultural soil samples were collected in selected locations in the states of Selangor, Negeri Sembilan and Melaka in Malaysia to identify and quantify veterinary antibiotic and hormone residues in the environment. The samples were analyzed using ultrasonic extraction followed by solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The broiler manure samples were found to be contaminated with at least six target analytes, namely, doxycycline, enrofloxacin, flumequine, norfloxacin, trimethoprim and tylosin. These analytes were detected in broiler manure samples with maximum concentrations reaching up to 78,516μgkg(-1) dry weight (DW) (doxycycline). For manure-amended agricultural soil samples, doxycycline and enrofloxacin residues were detected in every soil sample. The maximum concentration of antibiotic detected in soil was 1331μgkg(-1) DW (flumequine). The occurrence of antibiotics and hormones in animal manure at high concentration poses a risk of contaminating agricultural soil via fertilization with animal manure. Some physico-chemical parameters such as pH, total organic carbon (TOC) and metal content played a considerable role in the fate of the target veterinary antibiotics and progesterone in the environment. It was suggested that these parameters can affect the adsorption of pharmaceuticals to solid environmental matrices.

Concepts: Medicine, Concentration, Water pollution, Liquid chromatography-mass spectrometry, Phase diagram, Malaysia, Manure, Negeri Sembilan


Phosphate rock fertilization is commonly used in peatland restoration to promote the growth of Polytrichum strictum, a nurse plant which aids the establishment of Sphagnum mosses. The present study tested whether 1) phosphorus fertilization facilitates the germination of P. strictum spores and 2) biochar derived from local pig manure can replace imported phosphate rock currently used in peatland restoration. Various doses of biochar were compared to phosphate rock to test its effect directly on P. strictum stem regeneration (in Petri dishes in a growth chamber) and in a simulation of peatland restoration with the moss layer transfer technique (in mesocoms in a greenhouse). Phosphorus fertilization promoted the germination of P. strictum spores as well as vegetative stem development. Biochar can effectively replace phosphate rock in peatland restoration giving a new waste management option for rural regions with phosphorus surpluses. As more available phosphorus was present in biochar, an addition of only 3-9 g m(-2) of pig manure biochar is recommended during the peatland restoration process, which is less than the standard dose of phosphate rock (15 g m(-2)).

Concepts: Plant, Soil, Phosphate, Phosphorus, Moss, Manure, Mosses, Sphagnum


Phosphorus (P) is a critical nutrient used to maximize plant growth and yield. Current agriculture management practices commonly experience low plant P use efficiency due to natural chemical sorption and transformations when P fertilizer is applied to soils. A perplexing challenge facing agriculture production is finding sustainable solutions to deliver P more efficiently to plants. Using prescribed applications of specific soil microbial assemblages to mobilize soil bound-P to improve crop nutrient uptake and productivity has rarely been employed. We investigated whether inoculation of soils with a bacterial consortium developed to mobilize soil P, named Mammoth P™, could increase plant productivity. In turf, herbs, and fruits, the combination of conventional inorganic fertilizer combined with Mammoth P™ increased productivity up to twofold compared to the fertilizer treatments without the Mammoth P™ inoculant. Jalapeño plants were found to bloom more rapidly when treated with either Mammoth P. In wheat trials, we found that Mammoth P™ by itself was able to deliver yields equivalent to those achieved with conventional inorganic fertilizer applications and improved productivity more than another biostimulant product. Results from this study indicate the substantial potential of Mammoth P™ to enhance plant growth and crop productivity.

Concepts: Agriculture, Plant, Fertilizer, Nutrient, Soil, Nitrogen, Fruit, Manure