Discover the most talked about and latest scientific content & concepts.

Concept: Laser Doppler velocimetry


We assessed the cutaneous microcirculatory reactivity of a clinically unaffected skin region in patients with systemic sclerosis (SSc) compared to healthy controls by measuring transcutaneous oxygen saturation (TcPO2) and Laser Doppler flowmetry (LDF). Twelve consecutive patients with SSc and twelve healthy controls were subjected to TcPO2 monitoring and LDF during cuff-induced ischemia and reactive hyperemia in order to measure the skin oxygen tension and the microcirculatory blood flow. Mean minimal and maximal values of oxygen tension and blood flow, time to peak (TTP), and declining slopes after peaking (slope) were compared between patients with SSc and controls. Compared to the controls, TcPO2 values in SSc were similar during ischemia and diminished during reactive hyperemia, with shorter TTP, and a slower return to baseline (-60% vs. -58%, p = 1.000, +76% vs. +210%, p = 0.047, 137 s vs. 108 s, p = 0.028, -0.009%/s vs. -0.019%/s, p = 0.021, respectively). LDF values, however, did not differ significantly between patients with SSc and controls. Unaffected skin regions of SSc patients showed a significantly diminished postischemic vasodilatory reactivity when assessed by TcPO2 monitoring, but not by LDF, indicating that vasculopathy may represent an early mechanism in the onset of skin sclerosis. TcPO2 measurement may help to detect changes in the microcirculation in SSc with no skin affection.

Concepts: Hypoxia, Rheumatology, Laser Doppler velocimetry, Scleroderma, Oxygen, Systemic scleroderma, Measurement, Skin


We propose a motion-robust laser Doppler flowmetry (LDF) system that can be used as a non-contact blood perfusion sensor for medical diagnosis. Endoscopic LDF systems are typically limited in their usefulness in clinical contexts by the need for the natural organs to be immobilized, as serious motion artifacts due to the axial surface displacement can interfere with blood perfusion measurements. In our system, the focusing lens moves to track the motion of the target using a low-frequency reference signal in the optical data, enabling the suppression of these motion artifacts in the axial direction. This paper reports feasibility tests on a prototype of this system using a microfluidic phantom as a measurement target moving in the direction of the optical axis. The frequency spectra detected and the perfusion values calculated from those spectra show that the motion tracking system is capable of suppressing motion artifacts in perfusion readings. We compared the prototype LDF system’s measurements with and without motion feedback, and found that motion tracking improves the fidelity of the perfusion signal by as much as 87%.

Concepts: The Target, Tracking system, Laser Doppler velocimetry, Measurement, Perfusion, Tracking


Contrast ultrasound is a widely used clinical tool to obtain real-time qualitative blood flow assessments in the heart, liver, etc. Echocardiographic particle image velocimetry (echo-PIV) is a technique for obtaining quantitative velocity maps from contrast ultrasound images. However, unlike optical PIV, routine echo images are prone to non-uniform spatiotemporal variations in tracer distribution, making analysis difficult for standard PIV algorithms. This study introduces optimized procedures that integrate image enhancement, PIV and particle tracking velocimetry (PTV) to obtain reliable time-resolved 2D velocity distributions. During initial PIV analysis, multiple results are obtained by varying processing parameters. Optimization involving outlier removal and smoothing is used to select the correct vector. These results are used in a multi-parameter PTV procedure. To demonstrate their clinical value, the procedures are implemented to obtain velocity and vorticity distributions over multiple cardiac cycles using images acquired from four left ventricular thrombus (LVT) patients. Phase averaged data elucidate flow structure evolution over the cycle and are used to calculate penetration depth and strength of LV vortices, as well as apical velocity induced by them. Present data are consistent with existing[1] time-averaged minimum vortex penetration associated with LVT occurrence. However, due to decay and fragmentation of LV vortices, as they migrate away from the mitral annulus, in two cases with high penetration, there is still poor washing near the resolved clot throughout the cycle. Hence, direct examination of entire flow evolution may be useful for assessing risk of LVT relapse before prescribing anticoagulants.

Concepts: Vortex, Laser Doppler velocimetry, Velocimetry, Heart, Particle tracking velocimetry, Measurement, Fluid dynamics, Particle image velocimetry


Intracerebral hemorrhage (ICH) is the most feared complication in deep brain stimulation (DBS) surgery. The aim of the study was to evaluate patient safety and outcome using laser Doppler flowmetry (LDF) as guidance tool during DBS implantations.

Concepts: Heavy metal music, Medical terms, Safety, Implantation, Laser Doppler velocimetry, Cerebral hemorrhage, Deep brain stimulation


We propose a novel method for the robust, non-contact, and six degrees of freedom (6-DOF) motion sensing of an arbitrary rigid body using multi-view laser Doppler measurements. The proposed method reconstructs the 6-DOF motion from fragmentary velocities on the surface of the target. It is unique compared to conventional contact-less motion sensing methods since it is robust against lack-of-feature objects and environments. By discussing the formulation of motion reconstruction by fragmentary velocities, we show that at least three viewpoints are essential for 6-DOF motion reconstruction. Further, we claim that the condition number of the measurement matrix can be a measure of system accuracy, and numerical simulation is performed to find an appropriate system configuration. The proposed method was implemented using a laser Doppler velocimeter, a galvanometer scanner, and some mirrors. We introduce the methods for calibration, coordinate system selection, and the calculation pipeline, all of which contribute to the accuracy of the proposed system. For evaluation, the proposed system is compared with an off-line chessboard-tracking scheme of a 500 fps camera. Experiments of measuring six different motion patterns are demonstrated to show the robustness of the proposed method against different kinds of motion. We also conduct evaluations with different distances and velocities. The mean value error is less than 1.3 deg/s in rotation and 3.2 mm/s in translation, and is robust against changes in distance and velocity. For speed evaluation, the throughput of the proposed method is approximately 250 Hz and the latency is approximately 20 ms.

Concepts: Evaluation, Metrology, Laser Doppler velocimetry, Arithmetic mean, Distance, Numerical analysis, Kinetic energy, Measurement


The time taken to reach maximal haemostatic effect following local anaesthesia with epinephrine is generally believed to be <10 min. This is based on clinical experience and indirect measurements of perfusion using methods such as laser Doppler flowmetry and oxygen spectroscopy. However, the only study in which bleeding has been measured quantitatively in an intra-operative setting in humans showed that the full haemostatic effect was not achieved until 30 min after anaesthesia. The aim of this study was to determine the time taken to reach maximum haemostatic effect when using epinephrine for local anaesthesia in oculoplastic surgery.

Concepts: Ultraviolet, Laser Doppler velocimetry, Local anesthetic, Measurement, Local anesthesia, Anesthesia


The objective of this work is to define the conditions for improving the use of laser Doppler flowmetry (LDF) and to determine the limits for the use of this technique. This article systematically reviews the literature on the evaluation of oral soft tissue blood microcirculation by LDF. We analysed the available literature through October 2016 using the database resources Medline/PubMed, the Cochrane Oral Health Group Specialist Trials Register and the ISI Web of Knowledge. Several points emerged from this literature review The use of LDF involves specific constraints; however, the influence of different factors (temperature, tobacco, pressure etc.) must be adequately controlled when using LDF. LDF measurements of soft tissue within the oral cavity vary depending on the anatomical site. In dentistry, LDF can be used to track healing progress in periodontal surgery and to diagnose vascular flow changes in the connective tissue of mucosae covered by a removable prosthesis at an early stage prior to the onset of clinical inflammation signs.

Concepts: Dentistry, Collagen, Laser Doppler velocimetry, Surgery, Connective tissue, Hematology, Blood, Tissues


We present a novel non-contact system for monitoring the heart rate on human subjects with clothes. Our approach is based on vibrocardiography, and measures locally skin displacements. Vibrocardiography with a laser Doppler vibrometer already allows monitoring of this vital sign, but can only be used on bare skin and requires an expensive piece of equipment. We propose here to use an airborne pulse-Doppler ultrasound system operating in the 20-60 kHz range, and comprised of an emitter focusing the ultrasound pulses on skin and a microphone recording the reflected waves. Our implementation was validated in vitro and on two healthy human subjects, using simultaneously laser vibrocardiography and electrocardiography as references. Accurate measurements of the heart rate on clothed skin suggest that our non-contact ultrasonic method could be implemented both inside and outside the clinical environment, and therefore benefit both medical and safety applications.

Concepts: Cardiology, Medical signs, Pulse, Sound, Doppler effect, Laser Doppler velocimetry, Laser Doppler vibrometer, Measurement


In order to help a surgeon make the best decision, a more objective method of measuring ossicular motion is required.

Concepts: Doppler effect, Uncertainty, Laser Doppler velocimetry, Laser Doppler vibrometer, Measurement


To perform systematically a scoping review of the literature on commonly described continuous autoregulation measurement techniques in adult TBI. The goal was to provide an overview of methodology and comprehensive reference library of the available literature for each technique. Five separate small systematic reviews were conducted for each of the continuous techniques: pressure reactivity index (PRx), laser Doppler flowmetry (LDF), near infrared spectroscopy (NIRS) techniques, brain tissue oxygen tension (PbtO2), and thermal diffusion (TD) techniques. Articles from MEDLINE, BIOSIS, EMBASE, Global Health, Scopus, Cochrane Library (inception to December 2016) and reference lists of relevant articles were searched. A two-tier filter of references was conducted. The literature base identified from the individual searches was limited, except for PRx. The total number of articles utilizing each of the 5 searched techniques for continuous autoregulation in adult TBI were: PRx (28), LDF (4), NIRS (9), PbtO2 (10), and TD (8). All continuous techniques described in adult TBI are based on moving correlation coefficients. The premise behind the calculation of these moving correlation coefficients focuses on the impact of slow fluctuations in either MAP or CPP on some indirect measure of CBF, such as: intracranial pressure (ICP), LDF, NIRS signals, PbtO2 or TD CBF. The thought is the correlation between a hemodynamic driving factor, such as MAP or CPP, and a surrogate for CBF or cerebral perfusion sheds insight on the state of cerebral autoregulation. Both PRx and NIRS indices were validated experimentally against ‘golden standard’ static autoregulatory curve (Lassen curve) at least around lower threshold of autoregulation. PRx has the largest literature base supporting the association with patient outcome. Various methods of continuous autoregulation assessment are described within the adult TBI literature. Many studies exist on these various indices, suggesting an association between their values and patient morbidity/mortality.

Concepts: Laser Doppler velocimetry, Polynomial, Infrared spectroscopy, Spectroscopy, Traumatic brain injury, Near infrared spectroscopy, Intracranial pressure, Infrared