Concept: Hebrew numerals
Background Targeted temperature management is recommended for comatose adults and children after out-of-hospital cardiac arrest; however, data on temperature management after in-hospital cardiac arrest are limited. Methods In a trial conducted at 37 children’s hospitals, we compared two temperature interventions in children who had had in-hospital cardiac arrest. Within 6 hours after the return of circulation, comatose children older than 48 hours and younger than 18 years of age were randomly assigned to therapeutic hypothermia (target temperature, 33.0°C) or therapeutic normothermia (target temperature, 36.8°C). The primary efficacy outcome, survival at 12 months after cardiac arrest with a score of 70 or higher on the Vineland Adaptive Behavior Scales, second edition (VABS-II, on which scores range from 20 to 160, with higher scores indicating better function), was evaluated among patients who had had a VABS-II score of at least 70 before the cardiac arrest. Results The trial was terminated because of futility after 329 patients had undergone randomization. Among the 257 patients who had a VABS-II score of at least 70 before cardiac arrest and who could be evaluated, the rate of the primary efficacy outcome did not differ significantly between the hypothermia group and the normothermia group (36% [48 of 133 patients] and 39% [48 of 124 patients], respectively; relative risk, 0.92; 95% confidence interval [CI], 0.67 to 1.27; P=0.63). Among 317 patients who could be evaluated for change in neurobehavioral function, the change in VABS-II score from baseline to 12 months did not differ significantly between the groups (P=0.70). Among 327 patients who could be evaluated for 1-year survival, the rate of 1-year survival did not differ significantly between the hypothermia group and the normothermia group (49% [81 of 166 patients] and 46% [74 of 161 patients], respectively; relative risk, 1.07; 95% CI, 0.85 to 1.34; P=0.56). The incidences of blood-product use, infection, and serious adverse events, as well as 28-day mortality, did not differ significantly between groups. Conclusions Among comatose children who survived in-hospital cardiac arrest, therapeutic hypothermia, as compared with therapeutic normothermia, did not confer a significant benefit in survival with a favorable functional outcome at 1 year. (Funded by the National Heart, Lung, and Blood Institute; THAPCA-IH ClinicalTrials.gov number, NCT00880087 .).
Background Clostridium difficile is the most common cause of infectious diarrhea in hospitalized patients. Recurrences are common after antibiotic therapy. Actoxumab and bezlotoxumab are human monoclonal antibodies against C. difficile toxins A and B, respectively. Methods We conducted two double-blind, randomized, placebo-controlled, phase 3 trials, MODIFY I and MODIFY II, involving 2655 adults receiving oral standard-of-care antibiotics for primary or recurrent C. difficile infection. Participants received an infusion of bezlotoxumab (10 mg per kilogram of body weight), actoxumab plus bezlotoxumab (10 mg per kilogram each), or placebo; actoxumab alone (10 mg per kilogram) was given in MODIFY I but discontinued after a planned interim analysis. The primary end point was recurrent infection (new episode after initial clinical cure) within 12 weeks after infusion in the modified intention-to-treat population. Results In both trials, the rate of recurrent C. difficile infection was significantly lower with bezlotoxumab alone than with placebo (MODIFY I: 17% [67 of 386] vs. 28% [109 of 395]; adjusted difference, -10.1 percentage points; 95% confidence interval [CI], -15.9 to -4.3; P<0.001; MODIFY II: 16% [62 of 395] vs. 26% [97 of 378]; adjusted difference, -9.9 percentage points; 95% CI, -15.5 to -4.3; P<0.001) and was significantly lower with actoxumab plus bezlotoxumab than with placebo (MODIFY I: 16% [61 of 383] vs. 28% [109 of 395]; adjusted difference, -11.6 percentage points; 95% CI, -17.4 to -5.9; P<0.001; MODIFY II: 15% [58 of 390] vs. 26% [97 of 378]; adjusted difference, -10.7 percentage points; 95% CI, -16.4 to -5.1; P<0.001). In prespecified subgroup analyses (combined data set), rates of recurrent infection were lower in both groups that received bezlotoxumab than in the placebo group in subpopulations at high risk for recurrent infection or for an adverse outcome. The rates of initial clinical cure were 80% with bezlotoxumab alone, 73% with actoxumab plus bezlotoxumab, and 80% with placebo; the rates of sustained cure (initial clinical cure without recurrent infection in 12 weeks) were 64%, 58%, and 54%, respectively. The rates of adverse events were similar among these groups; the most common events were diarrhea and nausea. Conclusions Among participants receiving antibiotic treatment for primary or recurrent C. difficile infection, bezlotoxumab was associated with a substantially lower rate of recurrent infection than placebo and had a safety profile similar to that of placebo. The addition of actoxumab did not improve efficacy. (Funded by Merck; MODIFY I and MODIFY II ClinicalTrials.gov numbers, NCT01241552 and NCT01513239 .).
Our previously presented method for high throughput computational screening of mutant activity (Hediger et al., 2012) is benchmarked against experimentally measured amidase activity for 22 mutants of Candida antarctica lipase B (CalB). Using an appropriate cutoff criterion for the computed barriers, the qualitative activity of 15 out of 22 mutants is correctly predicted. The method identifies four of the six most active mutants with ≥3-fold wild type activity and seven out of the eight least active mutants with ≤0.5-fold wild type activity. The method is further used to screen all sterically possible (386) double-, triple- and quadruple-mutants constructed from the most active single mutants. Based on the benchmark test at least 20 new promising mutants are identified.
Safety of third-generation artificial turf in male elite professional soccer players in Italian major league
- Scandinavian journal of medicine & science in sports
- Published over 4 years ago
Our hypothesis is that there are no difference in the injury incidence on artificial turf and natural grass. During the 2011/2012 season, we recorded injuries which occurred to two Italian stadiums equipped with third-generation artificial turf during 36 games (391 players). Data were compared with the injuries which occurred in the same season in two stadiums equipped with natural grass (372 players). We recorded 43 injuries during the playing time (16.7 per 1000 h). About 23 (18.1 per 1000 h) injuries occurred on artificial turf, while 20 (15.2 per 1000 h) on the natural grass with no statistical differences P > 0.05. We recorded 10 (7.87 per 1000 h) contact and 13 (10.23 per 1000 h) non-contact injuries on artificial turf, while 5 (3.8 per 1000 h) contact and 15 (11.4 per 1000 h) non-contact injuries on natural grass P > 0.05. The overall relative risk was 1.15; 95% CI: 0.64-2.07). Our study demonstrates a substantial equivalence in injury risk on natural grass and artificial turf in elite professional soccer athletes during official matches.
Single and Combined Effects of Beetroot Crystals and Sodium Bicarbonate on 4-km Cycling Time Trial Performance
- International journal of sport nutrition and exercise metabolism
- Published over 3 years ago
When ingested alone, beetroot juice and sodium bicarbonate are ergogenic for high-intensity exercise performance. This study sought to determine the independent and combined effects of these supplements. Eight endurance trained (V̇O2max 65 mL·kg·min(-1)) male cyclists completed four x 4-km time trials (TT) in a double-blind Latin square design supplementing with beetroot crystals (BC) for 3 days (15 g.day(-1) + 15 g 1 h prior to TT, containing 300 mg nitrate per 15 g), bicarbonate (Bi 0.3 g·kg(-1) body mass [BM] in 5 doses every 15 min from 2.5 h prior to TT); BC+Bi or placebo (PLA). Subjects completed TTs on a Velotron cycle ergometer under standardized lab conditions. Plasma nitrite concentrations were significantly elevated only in the BC+Bi trial prior to the TT (1520 ± 786 nmol.L(-1)) compared to baseline (665 ± 535 nmol.L(-1), p = 0.02) and the Bi and PLA conditions (Bi: 593 ± 203 nmol.L(-1), p < 0.01; PLA: 543 ± 369 nmol.L(-1), p< 0.01). Plasma nitrite concentrations were not elevated in the BC trial prior to the TT (1102 ± 218 nmol.L(-1)) compared to baseline (975 ± 607 nmol.L(-1), p > 0.05). Blood bicarbonate concentrations were increased in the BC+Bi and Bi trials prior to the TT (BC+Bi: 30.9 ± 2.8 mmol.L(-1); Bi: 31.7 ± 1.1 mmol.L(-1)). There were no differences in mean power output (386 - 394 W) or the time taken to complete the TT (335.8 - 338.1 s) between any conditions. Under the conditions of this study, supplementation was not ergogenic for 4-km TT performance.
Effect of game format on heart rate, activity profile, and player involvement in elite and recreational youth players
- Scandinavian journal of medicine & science in sports
- Published about 6 years ago
The purpose of this study was to evaluate activity profile, aerobic load, and player involvement in two game formats of recreational and elite youth football for two age groups. A total of 152 youth players participated, with 45 U10 players playing 5v5 and 8v8 games, and 41 U13 players playing 8v8 and 11v11 (20 min) games. Activity profile, heart rate (HR), and technical actions were measured during all games using 10 Hz GPS, video filming, and HR monitors. For U10, no difference was found in total distance covered (1754 ± 237 vs 1771 ± 314 m, P = 0.650, d = 0.06), whereas mean HR (174 ± 10 vs 168 ± 12 bpm, P = 0.001, d = 0.59) and number of technical actions (65.1 ± 24.0 vs 36.9 ± 20.4, P 0.001, d = 1.27) were higher in 5v5 than in 8v8. For U13, lower total distance covered (1821 ± 325 vs 2038 ± 328 m, P < 0.001, d = 0.66) and higher number of technical actions (36.2 ± 14.9 vs 26.9 ± 14.1, P < 0.001, d = 0.64) were observed in 8v8 than in 11v11, with no difference in mean HR (170 ± 10 vs 171 ± 10 bpm, P = 0.679, d = 0.10). In conclusion, HR is high in youth football matches irrespective of the level of play and the game format. Playing with fewer players on smaller pitches results in minor changes to the physical loading but elevates the technical involvement of youth players both at elite level and recreational level.
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Accelerated shelf life tests have been conducted on very few shelf-stable products. The aim of this study was to reproduce quality changes occurring in neutral pudding packed in retort pouches over a one-year storage period at room temperature in approximately 100 days. The influence of headspace volume, packaging permeability, combination of packaging permeability and light, iron and copper on the quality changes of pudding throughout storage was investigated at the molecular and macroscopic scales. Analyses were performed from day 15 to 368 for control samples and from day 15 to 99 for pudding samples where one or more accelerating factors were applied.
AK-1NA and AK-2NA based on dibenzo-diazocine and anthracene moieties were designed and synthesized. Normalized UV-visible spectra of AK-1NA and AK-2NA in film state showed maximum absorption wavelength of 394 and 393 nm. PL spectra of AK-1NA and AK-2NA showed maximum emission wavelength of 429 and 444 nm. At a current density of 10 mA/cm2, OLED devices of AK-1NA and AK-2NA exhibited luminance efficiency of 2.39 and 1.50 cd/A, power efficiency of 1.01 and 0.81 lm/W. Also, OLED devices of AK-1NA and AK-2NA devices exhibited CIE(x, y ) of (0.16, 0.22) and (0.23, 0.42).
Methyl-substituted 2-nitrophenols are important components of “brown carbon” from biomass burning. Photolysis is their major gas-phase degradation pathway. To determine the extent of light absorptions by 4-methyl-2-nitrophenol and 5-methyl-2-nitrophenol, we obtained their absorption cross-sections in the 295-400 nm region by using cavity ring-down spectroscopy. Cross-section values for 4-methyl-2-nitrophenol were (1.01±0.07)×10-18, (5.72±0.39)×10-18, and (1.80±0.17)×10-20 cm2/molecule at 295, 345, and 400 nm, where errors quoted represent 1σ measurement uncertainty. Cross-section values for 5-methyl-2-nitrophenol were (9.04±0.77)×10-19, (5.89±0.54)×10-18 and (2.81±0.14)×10-20 cm2/molecule at 295, 345, and 400 nm. The HONO, NO2, and OH formation channels following 308 and 351 nm photolysis of methyl-2-nitrophenols were investigated. The OH quantum yields at 308 and 351 nm were obtained as the ratio of the OH concentration generated in pump/probe laser overlap region to the photon density absorbed by methyl-substituted 2-nitrophenol in the same region; they were 0.066±0.021 and 0.031±0.017 for 4-methyl-2-nitrophenol and 0.078±0.038 and 0.042±0.015 for 5-methyl-2-nitrophenol, where uncertainties represent 1σ precision. The average HONO quantum yields at 308 and 351 nm were 0.26±0.06 and 0.26±0.03 for 4-methyl-2-nitrophenol and 0.37±0.05 and 0.35±0.06 for 5-methyl-2-nitrophenol. Estimated OH production rates from photolyzing 10 pptv of 4-methyl- and 5-methyl-2-nitrophenol are 2.3×106 and 3.0×106 molecules•cm-3•s-1 at 16.9° zenith angle.