Suffering from giant size of objective lenses and infeasible manipulations of distant targets, telescopes could not seek helps from present super-resolution imaging, such as scanning near-field optical microscopy, perfect lens and stimulated emission depletion microscopy. In this paper, local light diffraction shrinkage associated with optical super-oscillatory phenomenon is proposed for real-time and optically restoring super-resolution imaging information in a telescope system. It is found that fine target features concealed in diffraction-limited optical images of a telescope could be observed in a small local field of view, benefiting from a relayed metasurface-based super-oscillatory imaging optics in which some local Fourier components beyond the cut-off frequency of telescope could be restored. As experimental examples, a minimal resolution to 0.55 of Rayleigh criterion is obtained, and imaging complex targets and large targets by superimposing multiple local fields of views are demonstrated as well. This investigation provides an access for real-time, incoherent and super-resolution telescopes without the manipulation of distant targets. More importantly, it gives counterintuitive evidence to the common knowledge that relayed optics could not deliver more imaging details than objective systems.
We exploit the inherent dispersion in diffractive optics to demonstrate planar chromatic-aberration-corrected lenses. Specifically, we designed, fabricated and characterized cylindrical diffractive lenses that efficiently focus the entire visible band (450 nm to 700 nm) onto a single line. These devices are essentially pixelated, multi-level microstructures. Experiments confirm an average optical efficiency of 25% for a three-wavelength apochromatic lens whose chromatic focus shift is only 1.3 μm and 25 μm in the lateral and axial directions, respectively. Super-achromatic performance over the continuous visible band is also demonstrated with averaged lateral and axial focus shifts of only 1.65 μm and 73.6 μm, respectively. These lenses are easy to fabricate using single-step grayscale lithography and can be inexpensively replicated. Furthermore, these devices are thin (<3 μm), error tolerant, has low aspect ratio (<1:1) and offer polarization-insensitive focusing, all significant advantages compared to alternatives that rely on metasurfaces. Our design methodology offers high design flexibility in numerical aperture and focal length, and is readily extended to 2D.
BACKGROUND: Optimizing operational parameters of the digital microscope system is an important technique to acquire high quality cytogenetic images and facilitate the process of karyotyping so that the efficiency and accuracy of diagnosis can be improved. OBJECTIVE: This study investigated the impact of condenser on cytogenetic image quality and system working performance using a prototype digital microscope image scanning system. METHODS: Both theoretical analysis and experimental validations through objectively evaluating a resolution test chart and subjectively observing large numbers of specimen were conducted. RESULTS: The results show that the optimal image quality and large depth of field (DOF) are simultaneously obtained when the numerical aperture of condenser is set as 60%~70% of the corresponding objective. Under this condition, more analyzable chromosomes and diagnostic information are obtained. As a result, the system shows higher working stability and less restriction for the implementation of algorithms such as autofocusing especially when the system is designed to achieve high throughput continuous image scanning. CONCLUSIONS: Although the above quantitative results were obtained using a specific prototype system under the experimental conditions reported in this paper, the presented evaluation methodologies can provide valuable guidelines for optimizing operational parameters in cytogenetic imaging using the high throughput continuous scanning microscopes in clinical practice.
This article demonstrates a method of tolerance reallocation via the Taguchi method for optical zoom lens design, which is based on the indicated specifications and corresponding tolerances derived from optical simulation in order to resolve the conventional dilemmas of performance between the modulation transfer function (MTF), relative illumination (RI), and distortion (DST) but also improve the performance without problems of tolerance. Generally speaking, the above refer to trade-offs between performance and tolerance of a kind which complicates modern optical design in high-quality products. In this paper, the Taguchi method of investigation was successfully adopted in seeking to balance MTF, DST, and RI so as to reallocate tolerance; therefore, we concluded that displays of multiperformance criteria can simultaneously give efficient help in tolerancing optimization in a way which approaches the exact manufacturing requirements. Without question, the proposed process could be copied when selecting other ways of evaluating image quality.
In this paper we present experimental studies on diffraction of V-point singularities through equilateral and isosceles right triangular apertures. When V-point index, also called Poincare-Hopf index (η), of the optical field is +1, the diffraction disintegrates it into two monstars/lemons. When V-point index η is -1, diffraction produces two stars. The diffraction pattern, unlike phase singularity, is insensitive to polarity of the polarization singularity and the intensity pattern remains invariant. Higher order V-point singularities are generated using Sagnac interferometer and it is observed that the diffraction disintegrates them into lower order C-points.
We report on the design of an off-axis three-mirror freeform telescope with a large field of view (FOV) based on an integration mirror (IM). This design is the continuation of the authors' previous work. Based on aberration theory, we established a suitable nonrelayed three-mirror-anastigmat initial configuration for integration mirror design. For an optical freeform surface, we analyzed the qualitative aberration correction ability of a x-y polynomial surface that can provide a simple, convenient, and user-friendly relationship between freeform surface term coefficients and aberrations and then applied the x-y polynomial surface on the tertiary mirror to improve the system optimization degrees of freedom. In an example with a focal length of 1200 mm, an F-number of 12, and a FOV of 1°×30°, the tolerance performance was analyzed, and the system presented a good imaging performance. In addition, the IM structure and opto-mechanics support structure were designed and analyzed. The confirmatory design results showed that the integration of the primary mirror and tertiary mirror can improve opto-mechanical properties judged by multiple criteria. In conclusion, the integration of the primary mirror and tertiary mirror not only offers alignment convenience as described previously but also improves system opto-mechanical properties in multiple perspectives. We believe this large linear FOV system based on IM has broad future applications in the optical remote sensing field.
Greek ladders with diffraction-limited array foci provide a probability to realize array imaging with equal intensity. Here, taking the ancient Theon sequence as an example, we design the optical structure and have measured the focusing properties by digital holography. Then, we verify the multiplanar imaging with different magnifications by experiment. The experimental results agree well with the theoretical analysis. In addition, bi-Fourier planes filtering technology is proposed to solve the problem of crosstalk between different imaging planes to further improve the imaging resolution. Therefore, we can freely design the focal length of the bifocal lens to achieve high-quality imaging at different resolutions. As a kind of amplitude-only diffractive lens, multifocal imaging provides a possibility of application in array biological imaging, ophthalmology, and an optical zoom system.
Fluorescence microscopy is a powerful approach for studying subcellular dynamics at high spatiotemporal resolution; however, conventional fluorescence microscopy techniques are light-intensive and introduce unnecessary photodamage. Light-sheet fluorescence microscopy (LSFM) mitigates these problems by selectively illuminating the focal plane of the detection objective by using orthogonal excitation. Orthogonal excitation requires geometries that physically limit the detection objective numerical aperture (NA), thereby limiting both light-gathering efficiency (brightness) and native spatial resolution. We present a novel live-cell LSFM method, lateral interference tilted excitation (LITE), in which a tilted light sheet illuminates the detection objective focal plane without a sterically limiting illumination scheme. LITE is thus compatible with any detection objective, including oil immersion, without an upper NA limit. LITE combines the low photodamage of LSFM with high resolution, high brightness, and coverslip-based objectives. We demonstrate the utility of LITE for imaging animal, fungal, and plant model organisms over many hours at high spatiotemporal resolution.
Unlike the electrostatic and electromagnetic lenses used in electron microscopy, most X-ray focusing optical systems have fixed optical parameters with constant numerical apertures (NAs). This lack of adaptability has significantly limited application targets. In the research described herein, we developed a variable-NA X-ray focusing system based on four deformable mirrors, two sets of Kirkpatrick-Baez-type focusing mirrors, in order to control the focusing size while keeping the position of the focus unchanged. We applied a mirror deformation procedure using optical/X-ray metrology for offline/online adjustments. We performed a focusing test at a SPring-8 beamline and confirmed that the beam size varied from 108 nm to 560 nm (165 nm to 1434 nm) in the horizontal (vertical) direction by controlling the NA while maintaining diffraction-limited conditions.
The iris, found in many animal species, is a biological tissue that can change the aperture (pupil) size to regulate light transmission into the eye in response to varying illumination conditions. The self-regulation of the eye lies behind its autofocusing ability and large dynamic range, rendering it the ultimate “imaging device” and a continuous source of inspiration in science. In optical imaging devices, adjustable apertures play a vital role as they control the light exposure, the depth of field, and optical aberrations of the systems. Tunable irises demonstrated to date require external control through mechanical actuation, and are not capable of autonomous action in response to changing light intensity without control circuitry. A self-regulating artificial iris would offer new opportunities for device automation and stabilization. Here, this paper reports the first iris-like, liquid crystal elastomer device that can perform automatic shape-adjustment by reacting to the incident light power density. Similar to natural iris, the device closes under increasing light intensity, and upon reaching the minimum pupil size, reduces the light transmission by a factor of seven. The light-responsive materials design, together with photoalignment-based control over the molecular orientation, provides a new approach to automatic, self-regulating optical systems based on soft smart materials.