SciCombinator

Discover the most talked about and latest scientific content & concepts.

RC Wang, Y Wei, Z An, Z Zou, G Xiao, G Bhagat, M White, J Reichelt and B Levine
Abstract
Aberrant signaling through the class I phosphatidylinositol 3-kinase (PI3K)-Akt axis is frequent in human cancer. Here, we show that Beclin 1, an essential autophagy and tumor suppressor protein, is a target of the protein kinase Akt. Expression of a Beclin 1 mutant resistant to Akt-mediated phosphorylation increased autophagy, reduced anchorage-independent growth, and inhibited Akt-driven tumorigenesis. Akt-mediated phosphorylation of Beclin 1 enhanced its interactions with 14-3-3 and vimentin intermediate filament proteins, and vimentin depletion increased autophagy and inhibited Akt-driven transformation. Thus, Akt-mediated phosphorylation of Beclin 1 functions in autophagy inhibition, oncogenesis, and the formation of an autophagy-inhibitory Beclin 1/14-3-3/vimentin intermediate filament complex. These findings have broad implications for understanding the role of Akt signaling and intermediate filament proteins in autophagy and cancer.
Tweets*
25
Facebook likes*
2
Reddit*
0
News coverage*
0
Blogs*
0
SC clicks
3
Concepts
AKT, Proteins, Cancer, Adenosine triphosphate, Oncology, Tumor suppressor gene, Cytoskeleton, Signal transduction
MeSH headings
-
comments powered by Disqus

* Data courtesy of Altmetric.com