SciCombinator

Discover the most talked about and latest scientific content & concepts.

I Younis, K Dittmar, W Wang, SW Foley, MG Berg, KY Hu, Z Wei, L Wan and G Dreyfuss
Abstract
Eukaryotes have two types of spliceosomes, comprised of either major (U1, U2, U4, U5, U6) or minor (U11, U12, U4atac, U6atac; <1%) snRNPs. The high conservation of minor introns, typically one amidst many major introns in several hundred genes, despite their poor splicing, has been a long-standing enigma. Here, we discovered that the low abundance minor spliceosome's catalytic snRNP, U6atac, is strikingly unstable (t½<2 hr). We show that U6atac level depends on both RNA polymerases II and III and can be rapidly increased by cell stress-activated kinase p38MAPK, which stabilizes it, enhancing mRNA expression of hundreds of minor intron-containing genes that are otherwise suppressed by limiting U6atac. Furthermore, p38MAPK-dependent U6atac modulation can control minor intron-containing tumor suppressor PTEN expression and cytokine production. We propose that minor introns are embedded molecular switches regulated by U6atac abundance, providing a novel post-transcriptional gene expression mechanism and a rationale for the minor spliceosome's evolutionary conservation. DOI:http://dx.doi.org/10.7554/eLife.00780.001.
Tweets*
4
Facebook likes*
2
Reddit*
0
News coverage*
8
Blogs*
1
SC clicks
1
Concepts
Gene, Cell nucleus, Messenger RNA, DNA, Gene expression, RNA, Spliceosome, RNA splicing
MeSH headings
-
comments powered by Disqus

* Data courtesy of Altmetric.com