Nano letters | 23 Nov 2012
F Gentile, M Moretti, T Limongi, A Falqui, G Bertoni, A Scarpellini, S Santoriello, L Maragliano, R Proietti Zaccaria and E Di Fabrizio
Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction doesn’t allow to get structural and functional information. Here we report on the direct imaging of double strand (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self aggregation process with Transmission Electron Microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.
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