SciCombinator

Discover the most talked about and latest scientific content & concepts.

C Zhang, Q Xue, J Hou, A Mohsin, M Zhang, M Guo, Y Zhu, J Bao, J Wang, W Xiao and L Cao
Abstract
In order to achieve rapid xylose utilization in the presence of acetate, improved yeast strains were engineered for higher bioethanol production. Firstly, a six-gene cluster including XYL1/XYL2/XKS1/TAL1/PYK1/MGT05196 was generated by using an in depth two-stage (glucose and xylose) transcription reprogramming strategy in an evolutionary adapted strain of CE7, resulting in two improved engineered strains WXY46 and WXY53. Through a combined screening of xylose and glucose stage-specific promoters between TCA/HSP and constitutive types respectively, WXY46 with the constitutive promoters showed much higher ethanol yield than that of WXY53 with the TCA/HSP promoters. Secondly, an optimized strain WXY74 was obtained by using more copies of six gene cluster, which resulted in higher ethanol yield of 0.500 g/g total sugars with acetate condition. At last, simultaneous saccharification and co-fermentation was perfmored by using the evolved WXY74 strain, which produced 58.4 g/L of ethanol from wheat straw waste, which outperformed previous haploid XR-XDH strains.
Tweets*
1
Facebook likes*
0
Reddit*
0
News coverage*
0
Blogs*
0
SC clicks
0
Concepts
-
MeSH headings
-
comments powered by Disqus

* Data courtesy of Altmetric.com