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AS Archambault, JA Carrero, LG Barnett, NG McGee, J Sim, JO Wright, T Raabe, P Chen, H Ding, EJ Allenspach, I Dragatsis, TM Laufer and GF Wu
Abstract
The activation, differentiation, and subsequent effector functions of CD4 T cells depend on interactions with a multitude of MHC class II (MHCII)-expressing APCs. To evaluate the individual contribution of various APCs to CD4 T cell function, we have designed a new murine tool for selective in vivo expression of MHCII in subsets of APCs. Conditional expression of MHCII in B cells was achieved using a cre-loxP approach. After i.v. or s.c. priming, partial proliferation and activation of CD4 T cells was observed in mice expressing MHCII only by B cells. Restricting MHCII expression to B cells constrained secondary CD4 T cell responses in vivo, as demonstrated in a CD4 T cell-dependent model of autoimmunity, experimental autoimmune encephalomyelitis. These results highlight the limitations of B cell Ag presentation during initiation and propagation of CD4 T cell function in vivo using a novel system to study individual APCs by the conditional expression of MHCII.
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Concepts
Protein, T helper cell, MHC class I, B cell, Adaptive immune system, Immune system, T cell receptor, Major histocompatibility complex
MeSH headings
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