OPEN Advanced science (Weinheim, Baden-Wurttemberg, Germany) | 25 Apr 2019
A Dilipkumar, A Al-Shemmary, L Kreiß, K Cvecek, B Carlé, F Knieling, J Gonzales Menezes, OM Thoma, M Schmidt, MF Neurath, M Waldner, O Friedrich and S Schürmann
Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label-free in vivo imaging in small animals via side-viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label-free endomicroscopy for longitudinal studies for the first time.
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