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ConvertingEscherichia coliinto an archaebacterium with a hybrid heterochiral membrane

OPEN Proceedings of the National Academy of Sciences of the United States of America | 21 Mar 2018

A Caforio, MF Siliakus, M Exterkate, S Jain, VR Jumde, RLH Andringa, SWM Kengen, AJ Minnaard, AJM Driessen and J van der Oost
Abstract
One of the main differences between bacteria and archaea concerns their membrane composition. Whereas bacterial membranes are made up of glycerol-3-phosphate ester lipids, archaeal membranes are composed of glycerol-1-phosphate ether lipids. Here, we report the construction of a stable hybrid heterochiral membrane through lipid engineering of the bacteriumEscherichia coliBy boosting isoprenoid biosynthesis and heterologous expression of archaeal ether lipid biosynthesis genes, we obtained a viableE. colistrain of which the membranes contain archaeal lipids with the expected stereochemistry. It has been found that the archaeal lipid biosynthesis enzymes are relatively promiscuous with respect to their glycerol phosphate backbone and thatE. colihas the unexpected potential to generate glycerol-1-phosphate. The unprecedented level of 20-30% archaeal lipids in a bacterial cell has allowed for analyzing the effect on the mixed-membrane cell’s phenotype. Interestingly, growth rates are unchanged, whereas the robustness of cells with a hybrid heterochiral membrane appeared slightly increased. The implications of these findings for evolutionary scenarios are discussed.
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Concepts
Lipid bilayer, Metabolism, Evolution, DNA, Lipid, Gene, Bacteria, Archaea
MeSH headings
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