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W Yang, L Carrillo-Reid, Y Bando, DS Peterka and R Yuste
Abstract
The simultaneously imaging and manipulating of neural activity in three-dimensions could enable the functional dissection of neural circuits. Here we have combined two-photon optogenetics with simultaneous volumetric two-photon calcium imaging to manipulate neural activity in mouse neocortex in vivo in 3D, while maintaining cellular resolution. Using a hybrid holographic approach, we simultaneously photostimulate more than 80 neurons over 150 μm in depth in cortical layer 2/3 from mouse visual cortex. We validate the usefulness of the microscope by photoactivating in 3D selected groups of interneurons, suppressing the response of nearby pyramidal neurons to visual stimuli. Our all-optical method could be used as a general platform to read and write activity of neural circuits.
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Concepts
Cerebrum, Nervous system, Cortical column, Visual perception, Pyramidal cell, Neuron, Brain, Cerebral cortex
MeSH headings
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