SciCombinator

Discover the most talked about and latest scientific content & concepts.

YS Bykov, M Schaffer, SO Dodonova, S Albert, JM Plitzko, W Baumeister, BD Engel and JA Briggs
Abstract
COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats, including COPI, have been extensively studied with in vitro reconstitution systems using purified components. In a previous paper (Dodonova et al., 2017), we determined a complete structural model of the in vitro reconstituted COPI coat. Here, we applied cryo-focused ion beam milling, cryo-electron tomography and subtomogram averaging to determine the native structure of the COPI coat within vitrified Chlamydomonas reinhardtii cells. The native algal structure resembles the in vitro mammalian structure, but additionally reveals cargo bound beneath β'-COP. We find that all coat components disassemble simultaneously and relatively rapidly after budding. Structural analysis in situ, maintaining Golgi topology, shows that vesicles change their size, membrane thickness, and cargo content as they progress from cis to trans, but the structure of the coat machinery remains constant.
Tweets*
2708
Facebook likes*
35
Reddit*
0
News coverage*
0
Blogs*
1
SC clicks
0
Concepts
Cell membrane, Secretion, Lysosome, Protein, Organelle, Cell, Golgi apparatus, Endoplasmic reticulum
MeSH headings
-
comments powered by Disqus

* Data courtesy of Altmetric.com