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Carcinogenesis is a complex process involving in genotoxic and non-genotoxic pathways. Carcinogenic potential of AgNPs has been predicted by genotoxic effects using several in vitro and in vivo models. However, there is no little information on non-genotoxic effects of AgNPs for carcinogenesis. In vitro cell transformation assay (CTA) can provide specific and sensitive evidence to predict the tumorigenic potential of a chemical, which cannot be supplied by genotoxicity testing. Therefore, we carried out CTA in Balb/c 3T3 A31-1-1 cells to evaluate the carcinogenic potential of silver nanoparticle (AgNPs). Colony forming efficiency (CFE) assay, and crystal violet (CV) assay were carried out to find cytotoxicity of AgNPs. Cytokinesis-block micronucleus assay (CBMN) and CTA in Balb/c 3T3 A31-1-1 cells were performed to predict in vitro carcinogenic potential of AgNPs. In CBMN assay, AgNPs (10.6 ug/mL) induced a significant increase of the micronucleus formation indicating that AgNPs had genotoxicity and could be an initiator for carcinogenesis. In CTA assay to assess carcinogenic potential of AgNPs, cells exposed to AgNPs for 72 h significantly induced morphological neoplastic transformation at all treated doses (0.17, 0.66, 2.65, 5.3, and 10.6 ug/mL) and Tf (transformation frequency) showed a significant increase in a dose-dependent manner. These results indicated that short-term exposure (72 h) to AgNPs had in vitro carcinogenetic potency in Balb/c 3T3 A31-1-1 cells.
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Toxicology, Cancer, Nanotechnology, Carcinogenesis, In vivo, In vitro, Mutagen, Oncology
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