SciCombinator

Discover the most talked about and latest scientific content & concepts.

V Chevelkov, K Giller, S Becker and A Lange
Abstract
In this report we present site-specific measurements of amide hydrogen-deuterium exchange rates in a protein in the solid state phase by MAS NMR. Employing perdeuteration, proton detection and a high external magnetic field we could adopt the highly efficient Relax-EXSY protocol previously developed for liquid state NMR. According to this method, we measured the contribution of hydrogen exchange on apparent (15)N longitudinal relaxation rates in samples with differing D2O buffer content. Differences in the apparent T1 times allowed us to derive exchange rates for multiple residues in the type III secretion system needle protein.
Tweets*
0
Facebook likes*
0
Reddit*
0
News coverage*
0
Blogs*
0
SC clicks
0
Concepts
Deuterium, Spin, Ferromagnetism, Measurement, Electron, Nuclear magnetic resonance
MeSH headings
-
comments powered by Disqus

* Data courtesy of Altmetric.com