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Necessity of Purification during Bacterial DNA Extraction with Environmental Soils

OPEN Environmental health and toxicology | 11 Aug 2017

HJ Lim, JH Choi and A Son
Abstract
Complexity and heterogeneity of soil samples have often implied the inclusion of purification steps in conventional DNA extraction for PCR assays. Unfortunately the purification steps are also time and labor intensive. Therefore the necessity of DNA purification was re-visited and investigated for a variety of environmental soil samples that contained various amounts of PCR inhibitors.
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Concepts
Gene expression, Labor, Soil, Laboratory techniques, Archaea, Polymerase chain reaction, Molecular biology, DNA
MeSH headings
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