A dark incubation period is important for agrobacterium-mediated transformation of mature internode explants of sweet orange, grapefruit, citron, and a citrange rootstock.
OPEN PloS one | 20 Oct 2012
M Marutani-Hert, KD Bowman, GT McCollum, TE Mirkov, TJ Evens and RP Niedz
Citrus has an extended juvenile phase and trees can take 2-20 years to transition to the adult reproductive phase and produce fruit. For citrus variety development this substantially prolongs the time before adult traits, such as fruit yield and quality, can be evaluated. Methods to transform tissue from mature citrus trees would shorten the evaluation period via the direct production of adult phase transgenic citrus trees.
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