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V Sokolova, D Kozlova, T Knuschke, J Buer, AM Westendorf and M Epple
Abstract
The uptake of calcium phosphate nanoparticles (diameter 120 nm) with different charge by HeLa cells was studied by flow cytometry. The amount of uptaken nanoparticles increased with increasing concentration of nanoparticles in the cell culture medium. Several inhibitors of endocytosis and macropinocytosis were applied to elucidate the uptake mechanism of nanoparticles into HeLa cells: Wortmannin, LY294002, nocodazole, chlorpromazine, and nystatin. Wortmannin and LY294002 strongly reduced the uptake of anionic nanoparticles, which indicates macropinocytosis as uptake mechanism. For cationic nanoparticles, the uptake was reduced to a lesser extent, indicating a different uptake mechanism. The localisation of nanoparticles inside the cells was investigated by conjugating them with the pH-sensitive dye SNARF-1. The nanoparticles were localised in lysosomes after 3 h of incubation.
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Concepts
Bacteria, Endocytosis, Growth medium, Flow cytometry, HeLa, Cell, Cell biology, Cell culture
MeSH headings
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