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Evaluation of recombinant MGL_1304 produced by Pichia pastoris for clinical application to sweat allergy

OPEN Allergology international : official journal of the Japanese Society of Allergology | 29 Jun 2015

T Kan, T Hiragun, K Ishii, M Hiragun, Y Yanase, A Tanaka and M Hide
Abstract
We previously identified MGL_1304 secreted by Malassezia globosa as a sweat antigen for patients with atopic dermatitis (AD) and cholinergic urticaria (ChU). However, purifying native MGL_1304 from human sweat or culture supernatant of M. globosa (sup-MGL_1304) is costly and time-consuming. Moreover, recombinant MGL_1304 expressed by using Escherichia coli (TF-rMGL_1304) needs a large chaperon protein and lacks the original glycosylation of yeasts. Thus, we generated a recombinant MGL_1304 by Pichia pastoris (P-rMGL_1304) and investigated its characteristic features.
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Concepts
Fungus, Atopy, Cholinergic urticaria, Gut flora, Endoplasmic reticulum, Yeasts, Protein, Escherichia coli
MeSH headings
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