SciCombinator

Discover the most talked about and latest scientific content & concepts.

V Garcia, SE Phelps, S Gray and MJ Neale
Abstract
Repair of DNA double-strand breaks (DSBs) by homologous recombination requires resection of 5'-termini to generate 3'-single-strand DNA tails. Key components of this reaction are exonuclease 1 and the bifunctional endo/exonuclease, Mre11 (refs 2-4). Mre11 endonuclease activity is critical when DSB termini are blocked by bound protein–such as by the DNA end-joining complex, topoisomerases or the meiotic transesterase Spo11 (refs 7-13)–but a specific function for the Mre11 3'-5' exonuclease activity has remained elusive. Here we use Saccharomyces cerevisiae to reveal a role for the Mre11 exonuclease during the resection of Spo11-linked 5'-DNA termini in vivo. We show that the residual resection observed in Exo1-mutant cells is dependent on Mre11, and that both exonuclease activities are required for efficient DSB repair. Previous work has indicated that resection traverses unidirectionally. Using a combination of physical assays for 5'-end processing, our results indicate an alternative mechanism involving bidirectional resection. First, Mre11 nicks the strand to be resected up to 300 nucleotides from the 5'-terminus of the DSB–much further away than previously assumed. Second, this nick enables resection in a bidirectional manner, using Exo1 in the 5'-3' direction away from the DSB, and Mre11 in the 3'-5' direction towards the DSB end. Mre11 exonuclease activity also confers resistance to DNA damage in cycling cells, suggesting that Mre11-catalysed resection may be a general feature of various DNA repair pathways.
Tweets*
1
Facebook likes*
0
Reddit*
0
News coverage*
0
Blogs*
0
SC clicks
3
Concepts
Adenosine triphosphate, BRCA1, Genetic recombination, Genetics, Gene, Homologous recombination, DNA repair, DNA
MeSH headings
Biocatalysis, DNA Breaks, Double-Stranded, DNA Repair, Endodeoxyribonucleases, Exodeoxyribonucleases, Meiosis, Models, Biological, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
comments powered by Disqus

* Data courtesy of Altmetric.com