Molecular analysis of meso- and thermophilic microbiota associated with anaerobic biowaste degradation.
OPEN BMC microbiology | 25 Jun 2012
J Ritari, K Koskinen, J Hultman, JM Kurola, M Kymäläinen, M Romantschuk, L Paulin and P Auvinen
Microbial anaerobic digestion (AD) is used as a waste treatment process to degrade complex organic compounds into methane. The archaeal and bacterial taxa involved in AD are well known, whereas composition of the fungal community in the process has been less studied. The present study aimed to reveal the composition of archaeal, bacterial and fungal communities in response to increasing organic loading in mesophilic and thermophilic AD processes by applying 454 amplicon sequencing technology. Furthermore, a DNA microarray method was evaluated in order to develop a tool for monitoring the microbiological status of AD.
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