Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining
Bioresource technology | 10 Nov 2012
D Xu, Z Gao, F Li, X Fan, X Zhang, N Ye, S Mou, C Liang and D Li
BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for lipid detection in the microalga Tetraselmis subcordiformis. A concentration of 0.28μgml(-1) and staining for 6min was optimal. Lipid bodies stained with BODIPY505/515 had a characteristic green fluorescence. Their volumes were determined using the sphere volume formula. Lipid accumulation under different nitrogen concentrations was analyzed. With an increase in NaNO(3) concentration from 0 to 240mg L(-1), the maximum algal concentration increased from 8.23±0.62 (×10(5) cells ml(-1)) to 1.61±0.13 (×10(6) cellsml(-1)), while the maximum volume of intracellular neutral lipid decreased from 9.78±1.77 μm(3)cell(-1) to 6.00±0.59μm(3)cell(-1). A comparison of the lipid contents measured by BODIPY 505/515 staining and the gravimetric method showed a positive correlation coefficient of R(2)=0.93. BODIPY 505/515 staining is a promising method in lipid quantitation in T. subcordiformis.
* Data courtesy of Altmetric.com