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Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Taraxacumcoreanum Nakaiis a dandelion native to Korea and is widely consumed as an edible and medicinal herb. The aerial part of T. coreanum (TC) has been used therapeutically as a diuretic and anti-inflammatory agent, but its mechanism of action has not yet been evaluated. AIM OF THE STUDY: To investigate the anti-inflammatory potential of a TCchloroform fraction(TCC) and its mechanisms of action in vitro and in vivo. MATERIALS AND METHODS: Isolated mouse peritoneal macrophages were stimulated in vitro with interferon-γ (IFN-γ) and lipopolysaccharide (LPS) in the presence or absence of TCC. The anti-inflammatory effects of TCC were assessed by measuring nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production, as well as expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), IκBα, phospho-IKK, mitogen-activated protein kinases (MAPKs), and signal transducer and activator of transcription (STAT1). The effects of TCC were tested in vivo by measuring cytokine production and survival in a mouse model of lethal septic shock. And the standard compounds of TC were analyzed by HPLC using a C18 column. RESULTS: Treatment of primary macrophages with TCC in vitro significantly inhibited all of the inflammatory parameters measured, including LPS-induced NO and PGE(2) production, iNOS and COX-2 expression, IκBα degradation, IKK phosphorylation, and MAPK and STAT1 activation. In a mouse model of LPS-induced septic shock, TCC inhibited the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, and increased survival by 83%.Standard compounds (gallic acid, syringic acid) of TC were qualified by HPLC analysis. CONCLUSIONS: TCC possesses potent anti-inflammatory activity in vitro and in vivo, which occurs at least partly through inhibition of proinflammatory signaling and mediator release. These results strongly support the therapeutic potential of TCC as an anti-inflammatory agent in vivo.
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Concepts
Mitogen-activated protein kinase, Immune system, Tumor necrosis factor-alpha, Nitric oxide synthase, Nitric oxide, Anti-inflammatory, Inflammation, Signal transduction
MeSH headings
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